use both PCIE x16 gen4 and gen3

pcie 4 gpu in pcie 3 slot
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A PCIe slot will not bottleneck the card But what CPU are you running? That might be a different story 6162021 EVGA_JacobF Nope

It clearly states its pcie4 for the gpu at x16 so it will be running at pcie4 all but x4 as the 6500xt is limited to that but working at its max

pcie gen 4 in gen 3 slot PCIe Gen for Professionals As mentioned earlier, PCIe is twice as fast as PCIe The same work which can be done by x16 slots of

pcie 40 in 30 slot Currently, even the fastest graphics cards from Intel, AMD, and Nvidia still utilize the outdated PCIe standard Sure, you can pair them with a PCIe Gen 5